They are participating in the London-Edinburgh-London Audax race from Sunday to Tuesday. And up to 1500 are expecting to pass through this region over a 48 hour period.
On the way north the route will pass through Longtown to Gretna, onto Ecclefechan and Lockerbie and up to Moffat.
Coming back down, it goes from Innerleithen to Ettrick then Eskdalemuir, Bentpath, Langholm, Rowanburn and Longtown.
Control points are being set up at Moffat Academy and at the Eskdalemuir Hub where the cyclists will be able to get their cards stamped, eat and sleep. They will both be manned by local volunteers.
The ride starts in Essex at 5 am on Sunday morning and it is expected to take about 116 hours.
London Edinburgh London is regarded as one of the UK’s greatest cycling challenges and includes 11,128m of climbing. Cyclists will be taking part from around the world.